Cell And Molecular Biology
My Key Skills:
Molecular Biology techniques: Genomic DNA/RNA, Plasmid isolation, RT-PCR and quantitative PCR, and Agarose gel Electrophoresis, Gel Elution, Preparation of competent Cells and Transformation.
Gene cloning and expression, Protein Isolation, Purification, Bradford Assay, His Tag, SDS-PAGE, Silver Staining.
Epigenetic related techniques: Slot dot blot/Dot Blot, ChIP using 5-hmC Antibody, 2D TLC,
Histone Methyltransferase Assays, Bisulfite conversion, oxidative bisulfite sequencing (oxBS) and Pyrosequencing.
Mammalian cell culture techniques: Routine maintenance of cell cultures, Media preparation, cell stock maintenance, Cell line transfection (HEK and HeLa Cells).
Chromatography and blotting techniques (HPLC, GC & AAS, western Blotting).
Worked as Project Assistant in the department of Molecular Biology at GENCORBIOLABS, Hyderabad from
1stAugust 2011 to 1stSeptember 2012.
CURRENTLY WORKING IN CDFD FORM 29TH APRIL TILL.As Project JRF-Junior Research Fellowship.
The Centre for DNA Fingerprinting and Diagnostics (CDFD) is an autonomous organization funded by the Department
of Biotechnology (DBT), Ministry of Science and Technology, Government of India.
PROJECT in M.Sc.
Title: Transgenic plant (Tobacco) using annexine gene by Agrobacterium mediated transformation. Location: # 2456 Manith Complex, 2ndfloor, 17th E-cross, 9thMain Banashankari 2ndStage,Bangalore-560-070.
Project summary: Plants are under constantly being subjected to both biotic and abiotic stress, which cause a reduction in yield, quality and growth rate. So we’ve dealt with some aspects of
Recombinant DNA Technology and molecular biology in combination with Plant tissue culture techniques to arrive at genetically engineered TOBACCO Plant.
Qualifications & Certifications
Biotechnology GPA 3
University of Kashmir