Fullerton - Microbiologist - Suffolk, VA, USA

Megan Fullerton

Suffolk, VA, USA



Work History

Research Scientist

BioControl Systems, Inc

May 2011 - July 2013

* Developed, Optimized and Validated methods for Assurance Genetic Detection System, GDS, (PCR based) Food Testing Kits for Salmonella, Listeria sp., Listeria monocytogenes, E. coli O157:H7, E. coli O104, Top 6 STEC, Top 7 STEC, Shiga like toxins and Cronobacter in a variety of different enriched foods and environmental swabs.
* Generated, Organized, and Analyzed Data from all food and environmental sample testing.
* Designed small validation experiments for initial assay testing.
* Began and maintained stability studies for all developed GDS assay components at three different storage temperatures for over time (up to two years.)
* Troubleshot Customer Issues relating to current products with various food enrichments or environmental swabs.
* Worked efficiently and effectively as a member of an interdisciplinary team within R&D.
* Responsible for collaborations between the R&D, QA and Production.
* Provided training for other internal departments on Assurance GDS kits and data analysis.
* Prepared master mixes and other reagents for GDS assays.

Gen-Probe Incorporated

January 2007 - October 2010

* Prepared wide range of bacterial/fungal cell cultures (broth and plate) and bacterial/fungal cell lysates. Examples include Salmonella, Mycoplasma, Mycobacterium, Aspergillus, MRSA, and VRE.
o Work performed in BSL2 and BSL3 laboratory setting.
* Prepared protozoan cell cultures and cell lysates.
o Examples include Trichomonas, and Pentatrichomonas
* Quantified bacterial and protozoan cell cultures
* Prepared microbiology area procedures (SOPs) for new techniques used in the lab.
* Performed DNA and RNA extractions of bacterial, viral and fungal samples.
* Identified various strains of bacteria using PCR, gene sequencing, Biolog and API.
* Used BLAST search and other software to confirm strain identifications.
* Performed molecular cloning of transcripts into E. coli
* Performed growth curve experiments for various strains of bacteria in presence of or absence of antibiotics.
* Performed Hybridization Probe Assay (HPA) on bacterial cell lysates to quantify bacteria using rRNAcopy levels.
* Prepared buffers for bacteria cell lysates.
* Prepared necessary media for bacteria, fungal and protozoan cell cultures.
* Prepared glycerol stocks and lyophilized pellets for long-term storage of strains.
* Knowledge of Real-Time Transcription Mediated Amplification (TMA).

Senior Research Associate

Gen-Probe Incorporated

October 2006 - January 2007

* Prepared bacterial cell cultures (broth and plate), and bacterial cell lysates.
* Performed growth curve experiments for various strains of bacteria in presence of antibiotic.
* Performed Hybridization Probe Assay (HPA) on bacterial cell lysates, and analyze data.
* Prepared buffers for bacterial cell lysis.
* Knowledge of Real-Time Transcription Mediated Amplification (TMA).

Laboratory Assistant

Department of Biological Sciences

May 2006 - August 2006

* Supervised and directed 2 graduate students and 2 undergraduate students working in the lab.
* Designed and performed experiments relating to the Lobster PaV1 grant (i.e. Restriction Enzyme digests, PCR, Sybrgreen Quantification assays, DNA extraction, etc.)

Qualifications & Certifications


Old Dominion University


Saint Mary's College of California

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