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De Alwis - Molecular Biology/ Microbiology - Los Angeles, CA, USA

Muditha De Alwis

Los Angeles, CA, USA

Services

Molecular Biology/ Microbiology

Summary:

Microbiology techniques (Aseptic/ isolation/ identification/culturing)
Plant Pathology techniques
Enzymatic manipulation of nucleic acids
Nucleic acid extraction & clean up
Gel electrophoresis (agarose & acrylamide gels)
Polymerase chain reaction (PCR)
Real Time PCR
Restriction fragment length polymorphism (RFLP) analysis
Terminal restriction fragment length polymorphism (TRFLP) analysis
Cloning
Sequencing
Plant tissue culture techniques
Sterilization Techniques
Preparation of reagents/media
Experience with laboratory equipment, materials, instruments and Autoclave
Knowledge of safety procedures in a laboratory environment

Work History

Graduate Research Assistant

Department of Plant Pathology, Washington State University, Pullman, WA

January 2009 - May 2011

Developed a non-fumigation/biological control method for apple replant
disease
Revealed the relationship between rootstock genotype and biocontrol agent
to persist in the apple rhizosphere and provide disease control

Research Officer

Department of Forestry, Ministry of Environment & Natural Resources, Sri Lanka

April 2008 - December 2008

Carried out both lab and the field research to find cause of diseases of plants
and trees

Research Assistant

Department of Botany, University of Kelaniya, Sri Lanka

October 2002 - December 2007

Investigated potential use of decomposing rice straw as an organic soil
amendment
Develop laboratory protocols for undergraduate students
Assist in organizing laboratory session for undergraduate students majoring molecular biology

Teaching Assistant

Department of Botany, University of Colombo, Sri Lanka

March 2001 - July 2002

Developed protocol for micro propagation of rose
Conducted laboratory classes for undergraduate students majoring plant science

Qualifications & Certifications

Master of Science in Plant Pathology

Washington State University, Pullman, WA

Master of Philosophy in Botany

University of Kelaniya, Kelaniya

Bachelor of Science

University of Colombo, Sri Lanka

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