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Adlam - Immunology - Newark, NJ, USA

Matthew Adlam

Newark, NJ, USA

Services

Immunology

Summary:

Skilled Immunologist, 18 yrs experience

Work History

Consultant

From March 2011

, New York City, New York.
• Provided direct consultation with a professor at Davidson College N.C. with regards to CD4 gene expression and BAC recombineering.
• Provided direct hands-on help with BAC recombineering.
• Remained current with applicable literature.

Research Associate III

University of Medicine and Dentistry of New Jersey

October 2009 - March 2011

, New Jersey.
• Ran experiments to elucidate the in vivo role of IL-12 in T cell memory.
• Utilized an in vivo murine toxoplasmosis infection model, qRT-PCR, FACs staining and sorting of T cells.
• Competitive bone marrow transplantion into immunodeficient (SCID/NOD) mice.
• Generation of a BAC based transgene.
• Maintained GLP data records, collating and analyzing data and communicating data to team and departments. Remained current with applicable literature. Mentor other lab members and fellow scientists..

Research Scientist

Department of Inflammation, Amgen Inc

May 2003 - September 2007

• Identified putative targets, formed and managed a team to investigate the target.
• Participated as in vivo and in vitro team representative.
• Generated Hu/SCID chimeric mice by transferring human umbilical cells into immunodeficient mice.
• Utilized murine in vivo inflammation models (air pouch, collagen induced arthritis (rat), and prostaglandin driven ear inflammation) to test putative drug leads.
• Directed junior members to run assays.
• Maintained GLP data records, collating and analyzing data and communicating data to team and departments. Remained current with applicable literature. Mentor other lab members and fellow scientists.

Hemangioblast development

Proficient Methodologies
In vivo models
Models of inflammation (air pouch, PGE2 induced inflammation, collagen induced arthritis). Genetic manipulation models using transgenesis and targeted gene disruption. Transgene and "knock -out" construction, microinjection, line screening and propagation, ex vivo / in vivo analysis. Highly experienced with FACs to study lymphopoiesis and the mature immune function. Short and long term models of T cell development and function in response to intracellular pathogens (Toxoplasmosis gondii) and immunogens.
In vitro models
Genetic manipulation of embryonic stem cells and subsequent analysis. In vitro lymphopoiesis from ES cells. Transient and stable transfection of both reporter and transcriptional inducer constructs. T cell proliferation assays. B cell class switching. Development and stringent validation of novel high throughput cell based assays. Rectification of high throughput cell based assays.
Molecular and Protein
Nuclear extract purification (small and large scale), EMSA, foot-printing, immunoprecipitation (co-IP, co-IP UV x-link, exogenous protein purification (mammalian and bacterial), hybridoma propagation and mAb purification. mAb labeling. Extensive FACs phenotyping. Large scale FACs based cell purification of rare populations. Superantigen production and utility. Glycosylation biology
Transcriptional control element trapping by genetics. Cloning, sub-cloning (plasmid, cosmid, BAC in mammalian and bacterial cells), most PCR based methodologies, manual sequencing, foot-printing, transgene and knock-out construction, southern, western and northern blotting, RNA and DNA preparation, large scale screening, transgene microinjection, ES cell transgenesis, transfection (reporter and inducer). Development and validation of novel high throughput protein based assays.

Post-doctoral fellow

Mount Sinai School

January 2002 - May 2003

• Assessed function of mMix1 by use of genetically modifying and differentiating murine ES cells. Utilized FACs, and various biochemical assays to assess mMix1 function
• Maintained GLP data records, collated and communicated data with group and department. Remained current with applicable literature. Mentor other lab members and fellow scientists.

"Hemangioblast development"

Post-doctoral Fellow

Department of Microbiology, Columbia University, College

September 2001 - January 2002

• Assessed function of CD4 gene control elements during T cell development by personally generating minigene transgenic mice (personally generated more than 500 lines of mice), as assessed by flow cytometry, southern blot, PCR and in situ hybridization (see Immunity paper as example).
• Maintained mouse colony of approximately 600 cages, maintaining collection of transgenic lines by continuous breeding and screening.
• Utilized various biochemical techniques to probe the proteins and protein interactions during thymocyte development (see list below).
• Transient transfection In vitro tissue culture assays of CD4 element response to exogenous transcription factors or signaling proteins
• Maintained GLP data records, collated and communicated data with group and department. Remained current with applicable literature. Mentor other lab members and fellow scientists.

Post doc

College of Physicians and Surgeons

September 1996 - September 2001

• As above. Assessed CD4 gene control by use of transgenics, in vitro tissue culture and biochemistry assays.
• Fetal thymic organ culture.
• Maintained mouse colony and transgenic lines.
• Maintained GLP data records, collating and analyzing data and communicating data to team and departments. Remained current with applicable literature. Mentor other lab members and fellow scientists.

Research technician

Department of Microbiology, Columbia University, College

June 1994 - September 1996

• As above. Assessed CD4 gene control by use of transgenics, in vitro tissue culture and biochemistry assays.
• Maintained lab infrastructure such as equipment, supplies.
• Maintained mouse colony and transgenic lines.
• Maintained GLP data records, collating and analyzing data and communicating data to team and departments. Remained current with applicable literature. Mentor other lab members and fellow scientists.

Research technician, Department of Molecular Medicine

Auckland School

December 1991 - April 1994

of Medicine. New Zealand
• Investigated streptococcus superantigens utilizing primary human lymphocyte culture followed by TcR phenotyping.
• Cloning of a novel streptococcus pyogenes superantigen.
• Maintained GLP data records, collating and analyzing data and communicating data to team and departments. Remained current with applicable literature. Mentor other lab members and fellow scientists.

Teaching assistant and Mentor, Department of Physiology

Victoria University

February 1991 - November 1991

of Wellington, New Zealand
• Taught 2nd year university students animal surgery in wet lab experiments utilizing rat, rabbit and toad (both survival and terminal surgery)
• Held tutorial classes for 2nd year university students.

Internship

Wallacevile Animal Research Institute

November 1991 - February 1992

, Wallaceville, New Zealand.
• Collected and processed blood from flocks of sheep to investigate genetics of helminthe resistance.
• ELISA assays to assess anti-helminthe antibodies.

Part-time animal technician

Victoria University

February 1989 - November 1991

of Wellington, New Zealand.
• Maintained rat colony and assessed oestrous cycles of rats, used to investigate the role of prolactin in pup fostering.

Grants and Awards
2009 Competitive core facility grant for molecular biology.
2009 Competitive core facility grant for mouse genetics.

Qualifications & Certifications

Integrated Program

College of Physicians and Surgeons, Columbia University

Integrated Program

College of Physicians and Surgeons, Columbia University

Cellular Molecular and Biophysical Studies

College of Physicians and Surgeons, Columbia University

Biochemistry and Physiology

Victoria University of Wellington

St Patricks, Silverstream, NZ

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