Kolachalam - Neuroscientist - Bangalore, KA, India

Manish Kolachalam

Bangalore, KA, India



  • Full time
  • Part time
  • One time
  • Contract
  • Temp

Work History

Master of Philosophy

National Centre for Biological Sciences, Bangalore, India


Patch clamp electrophysiology
In vitro field electrophysiology
Retrograde tracing
C-fos immunohistochemistry
Confocal microscopy


Attended the Australian Course in Advanced Neuroscience (ACAN)
Attended the Harvard-NCBS course on optogenetics


Teaching assistantships in Neurobiology and Cell biology


Effects of stress on amygdalar neurons projecting to the medial prefrontal cortex

I developed tools that would allow one to identify neurons projecting to a particular brain area and study them using patch clamp electophysiology in rat brain slices. We were interested in the manner in which amygdalar neurons projecting to the medial prefrontal cortex are affected by stress. I combined retrograde tracing with patch clamp electrophysiology in acute amygdalar slices enabling one to study changes in projection specific neuronal populations. This strategy was also extended to study these neuronal populations using immediate early gene immunohistochemistry.

Effects of chronic stress on Intrinsic plasticity in the hippocampus

I was involved in a study trying to understand the effects of chronic immobilization stress on E-S potentiation, a form of intrinsic plasticity, in the CA3 region of the hippocampus. Though we did not find any effects of stress on this form of plasticity, in the process I obtained extensive experience in field electrophysiology.

Qualifications & Certifications

Master of Philosophy, Neuroscience

National Centre for Biological Sciences

Master of Science, Biotechnology

Maharaja Sayajirao University of Baroda

Bachelor of Science, Biotechnology

Hislop College, RTM Nagpur University

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