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Ganapathy - Biochemist - Lubbock, Texas, USA

Kulothungan Ganapathy

Lubbock, Texas, USA


I am a Biochemist, specialized in molecular biology and microbiology with expertise in PCR, site-directed mutagenesis, recombinant DNA technology, molecular biomarkers, enzymology, protein purification, mass spectrometry and drug efficacy testing in human pathogens (prokaryotes and eukaryotes),. Ability to do multitasking and work independently. Strong motivations towards research and development. Excellent Interpersonal and analytical skills.

I have worked intensively in the area of membrane protein engineering, purification, characterization and mechanistic enzymology. DNA, RNA and protein isolation and sequence analysis, enzyme steady-state kinetics including radioactive assays, protein drug targeting and inhibition studies, polymerase chain reaction, electrophoresis and mass spectrometry. I am also experienced in bioinformatics, performing sequence database search and analysis and protein structure identification. I am proficient in using scientific software packages like Vector NTI, Sigma Plot Enzyme Kinetic Module, ChemBio Office. Chromeleon, ChemStation, Xcalibur, Mascot, Proteome Discoverer, PinPoint.
I have experience in working with human pathogens including BL2 organisms and large scale production of various recombinant and wild-type strains across kingdoms for protein isolation for inhibitor study and sterol isolation. I have trained several undergraduate students in my research group during my doctoral program. I was a graduate assistant for Instrumentation in the Biochemistry Department which includes technical maintenance and teaching.



  • Full time
  • Part time
  • One time
  • Contract
  • Temp

Work History

Post Doctoral Research Associate

Texas Tech University

May 2010 - May 2010


1. Jialin Liu1, Kulothungan Ganapathy1, Chizaram A. Nwogwugwu and W. David Nes. Parasite sterol biosynthesis: Effect of substrate features and mutagenesis of active site tyrosine residues on the reaction course catalyzed by Trypanosoma brucei sterol C24-methyltransferase BJ (manuscript under preparation)

2. Kulothungan Ganapathy1, Ragu Kanagasabai1, Thuy Thi Minh Nguyen and W. David Nes*. Purification, Characterization and Inhibition of Sterol C24-Methyltransferase from Candida albicans, Archives of Biochemistry and Biophysics 505 (2011) 194-201

3. Maristela Pereira, Zhiong Song, Ludier Kesser Santos-Silva, Mathew H. Richards, Thi Thuy Nguyen, Jialin Liu, Kulothungan Ganapathy and W.David Nes*. Cloning, Mechanistic and Functional Analysis of a Fungal Sterol 24C-Methyltransferase Implicated in Brassicasterol Biosynthesis, Biochemica et Biophysica Acta 1801(10) (2010): 1163-74

4. W.David Nes*, Wenxu Zhou, Kulothungan Ganapathy , Jialin Liu, Rit Vatsyayan, Swetha Chamala, Keven Hernandez, Mayra Miranda. Sterol 24-C-methyltransferase: An enzymatic target for the disruption of ergosterol biosynthesis and homeostasis in Cryptococcus neoformans, Archives of Biochemistry and Biophysics 481 (2009): 210-218.

5. Kulothungan Ganapathy, Christopher W.Jones, Camille M. Stephens, Rit Vatsyayan, Julie A. Marshall and W. David Nes*. Molecular Probing of the Saccharomyces cerevisiae Sterol 24-C-Methyltransferase Reveals Multiple Amino Acid Residues Involved with C2-transfer Activity, Biochemica et Biophysica Acta 1781 (2008): 344-351

6. M Gomes, G Kulothungan, S S Mehetre and S Eapen*. Analysis of genetic relatedness in some Gossipium species by RAPD, Indian Journal of Biotechnology 3 (2004): 41-46

Graduate student (Ph.D)

Texas Tech University

September 2004 - March 2010

Dissertation Title:

Qualifications & Certifications


Texas Tech University

Master of Science

University of Madras

Bachelor of Science

University of Madras

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