Hsiao - Antibody / Protein Engineering - Tewksbury, Massachusetts, USA

Ku Chuan Hsiao

Tewksbury, Massachusetts, USA


Antibody / Protein Engineering

  • Full time
  • Part time
  • One time
  • Contract
  • Temp

Work History

Sr Scientist

Biogen Idec Hemophilia

October 2007 - April 2011

• Head of phage display technology: managing all the phage activities in a timely fashion for the company. Responsible for leading, designing and conducting of critical experiments that included the design and construction of libraries, as well as the subsequent panning and characterization, affinity maturation and optimization based on the interaction-activity relationships of the binders.
• Developed and established competition immunoassays for the phage hits.
• Identified and characterized peptide agonists/antagonists for ligands and receptors that are important for hemophilia, anemia, and multiple sclerosis using both commercial and in-house phage libraries.
• Identified new targets in hematology related areas, and used phage display to initiate new research projects that would result in patent applications for the company.
• Head of the automation system for the identification of phage hits using a high throughput format; identified and purchased the $250k Agilent Bravo automation system.
• Provided advice on projects to the managements and nurtured the professional growth of direct reports.
• Started a journal club for the company.


Idexx Laboratories

August 2003 - October 2007

• In charged of phage display Fab and peptide libraries’ design/construction, the subsequent panning, as well as the characterization and affinity maturation/optimization of the binders.
• Managed projects in a timely fashion for reagent/assay development and validation, supplying other groups with reagents to be used in assays: Cloning and expressing structural and non-structural proteins of pathogen, e.g., FIV, hog cholera, avian influenza, E. chaffeensis, toxocara, canine and feline pancreatic lipases and others.
• Designed, developed and optimized binding and competition immunoassays (ELISAs) in various phage related programs.
• Identified peptides that differentiate between closely related Anaplasma species using phage display technology.
• Identified diagnostic and therapeutic Abs for heartworm using specially designed phage display libraries.
• Purified in-house expressed proteins using various biochemical and chromatographical means (e.g., AKTA).
• Design/construction of IP-free expression vectors.
• Developed affinity tags for expressed proteins.
• Prepared SOPs and legal documents in support of IP filings.
• In charge of protein production of Abs in 20 liter fermentors for clinical studies.
• Provided advice on projects to the managements and nurtured the professional growth of direct reports.

Sr Scientist

DGI Biotechnology

January 1996 - October 2002

• Developed and managed/ supervised the operation of all the cell-based assays and their validation of the drug leads for all DGI projects.
• Identified, characterized and affinity matured as well as optimized peptide agonists/antagonists for proprietary cancer and immunological ligands and receptors using DGI’s phage libraries.
• Developed and optimized competition ELISAs to help rank discovered drug leads.
• In depth studies in structure-activity relationships as well as signal transduction initiated by these peptides; involved in the discovery of small molecules for these ligands/receptors.
• Identified areas of research and initiated many projects using phage display which resulted in patent applications.
• Initiated and managed external collaborations with Lombardi Cancer Center of Georgetown University.
• Key scientist in obtaining DGI’s first two deals with Novo Nordisk on insulin and IGF projects using phage display technology.
• Successfully isolated both agonists and antagonists for insulin and IGF receptors, TNF receptors, Fas…etc. from phage peptide libraries and further developed cell-based activity assays for these scanners.
• Led a team of 3 Ph.D. scientists and their associates, and successfully developed genomic/semi-synthetic single chain antibody phage display libraries.
• Developed green fluorescent protein constructs. Successfully evaluated the feasibility of eukaryotic expression systems for DGI, systems including: yeast (Pichia), insect (baculovirus) and mammalian (COS and CHO).
• Prepared SOPs, SBIRs and legal documents in support of IP filings.
• Provided advice on projects to the managements and nurtured the professional growth of direct reports.
• Started a journal club for the company.


Idexx Laboratories

May 1994 - January 1996

• Allergy therapeutics (expression of antibodies, antibody fragments, fusion proteins in mammalian cell culture systems).

Post doc

Bristol Myers Squibb Pharmaceutical Research Institute

May 1990 - May 1994

• Antibody Engineering (hybridoma generation, chimerization, humanization and biosynthesis of recombinant Abs).

Qualifications & Certifications

Bachelor of Sciences

University of Wisconsin - Eau Claire

MS - Biological Sciences

University of Wisconsin-Milwaukee

MS - Microbiology

University of Illinois at Urbana-Champaign

PhD - Biophysics and Physiology

University of Illinois at Urbana-Champaign

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