Animal Cell Culture
Media preparation and filtration
Maintenance of cell lines
Cell culture based studies (MTT assay, growth curve studies)
Bangalore Biotech Labs Pvt. Ltd. (BIOZEEN)
Worked as DBT's BCIL trainee at Bangalore Biotech Labs Pvt. Ltd. (BIOZEEN) for 6 months.
Key Expertise and skills:
❖ Downstream Processing: Spectrophotometer, Microfilteration, UltraFilteration, Diafilteration, chromatography techniques (ion exchange, affinity, hydrophobic and gel filtration), Agarose Gel Electrophoresis (AGE), Poly Acralamide Gel Electrophoresis (PAGE), SDS-PAGE.
❖ Cleaning, Sterilization & Filtration: Validation of autoclave and DHS (dry heat sterilization), ESIP (empty vessel sterilization in place) of fermenter, FSIP (full vessel sterilization in place) of fermenter, CIP (clean in place) of filters, Integrity testing of filters (water intrusion test, bubble point test, diffusion test).
❖ Animal Cell Culture: Media preparation and filtration, Sub culturing, Cell enumeration, Maintenance of cell lines, Cell culture based studies (MTT assay, growth curve studies), Cryopreservation, Giemsa staining, Karyotyping, Stem cell techniques like differentiation and characterization and IHC.
❖ Molecual Biology and Genetic engineering: Basic Recombinant DNA Techniques (Isolation, purification and characterization of proteins, DNA and RNA). Conventional PCR and RT PCR, Dialysis, Restriction digestion, Gene cloning, Expression studies, Blotting techniques.
❖ Enzyme technology: Determination of enzyme activity, Specific activity, km values, enzyme assays, Analysis of different parameters effect (ph, Temperature, Enzyme concentration, Substrate concentration) on enzyme activity, Estimation of protein concentration.
❖ Microbial Fermentation: Isolation techniques (streak plate, spread plate, pour plate), Serial dilution, shake flask optimization (media, ph, inoculum optimization), Lab scale fermentation, Pilot scale fermentation, Determination of KLa.
✓ Fair knowledge on MS-Office 2007, Windows7.
✓ Done course in HTML, C languages.
✓ Good Typing Skills
✓ Good PDF editing skills.
Qualifications & Certifications
Sri Venkateswara Institute of Medical sciences
Sri Venkateshwara University
Sri Vidyaranya High school
Sri Chaitanya Bharathi junior college
UNIVERSITY / BOARD
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