Altaweel - Biotechnologist - Winnipeg, MB, Canada

Khaled Altaweel

Winnipeg, MB, Canada

Services

Biotechnologist

Summary:

Gene Expression Analysis
 Transcriptome profiling of resistant genes expressed under stress conditions using qRT-PCR
 Sequence homology searches and sequence-deposit in GenBank (NCBI)

Gene Manipulation
 Extensive experience with standard molecular biology enzymatic reactions, ligations, PCR, DNA cloning, sequencing, western blot analysis, and other molecular experiments
 Gene identification, isolation, cloning, transformation and molecular characterization
 Subtracted cDNA library construction
 cDNA library construction

Genetic Engineering
 Agrobacterium- and Biolistic- mediated transformation
 Molecular characterization of transgenic plants using molecular biological techniques
 Quantitative detection of proteins (post-translational modification)
 Plasmid DNA isolation and transformation of bacteria for preparation of a construct (vector)
 Bacteria culturing, selection of transformed cells and maintenance of cultures
 Preparation of transformation-competent cells
 Genetically Modified Organisms (GMOs)-detection by PCR- & protein-based methods
 Field evaluation of transgenic plants for agronomically important traits

Tissue Culture
 Tissue culture and micrografting techniques
 Meristem isolation and culture
 Regeneration survey (evaluation of plant genotypes for response to tissue culture)
 Standardization plant tissue culture protocol
Interpersonal/Communication
 Supervised graduate and undergraduate students within the laboratory setting, created a positive, well integrated environment crossing all linguistic, cultural and ethnic backgrounds
 Established an environment that fostered students striving for excellence by focusing on what they had done well, encouraging improvement in constructive and positive ways
 Consistently demonstrated accuracy, precision and a high degree of organization in all scientific experimentation, research and documentation
 Primary focus in the work is always the quality of the data

Work History

Faculty of Pharmacy, University of Manitoba, Canada

Voluntary Work Studies

(2013-till now)

Project title: Gene expression of nerve growth factor (NGF) and methyl CpG binding protein 2 (MeCP2) in the nervous system. Faculty of Pharmacy, University of Manitoba, Canada.
• Quantitative real-time PCR (qRT-PCR) of NGF and MeCP2 under disease (experimental autoimmune encephalomyelitis, EAE) for both Dorsal Root Ganglia (DRG) and Spinal Cord (SC).

Research Associate Studies

Plant Science Department

Project title: Transcriptome profiling of susceptible and resistant wheat infected by Fusarium graminearum. Plant Science Department, Faculty of Agricultural & Food Sciences, University of Manitoba, Canada.
• Quantitative transcript accumulation and gene expression analysis using real-time PCR.
• Transcriptome profiling of resistant genes expressed under stress conditions.
• Homology searching analysis of DNA sequence and deposit in GenBank (NCBI).

Plant Science Department, Faculty

Post-Doc Studies

(2009-2010)

Project title: Molecular identification, characterization, and cloning of wheat resistance genes expressed under different isolates of Fusarium graminearum. Plant Science Department, Faculty of Agricultural & Food Sciences, University of Manitoba, Canada.
• Extensive experience with standard molecular biology enzymatic reactions, primer designing, PCR, DNA cloning and sequencing.
• Gene identification, isolation, cloning and transformation.
• Subtracted cDNA library construction.
• cDNA library construction.
• Differential genes expression methods: Differential Display RT-PCR (DDRT-PCR), Suppressive Subtractive Hybridization (SSH).

PhD. Studies

Osaka Prefecture University

Project title: A bacterial transgene for catalase protects translation of D1 protein during exposure of salt-stressed tobacco leaves to strong light. Graduate School of Life & Environmental Sciences, Osaka Prefecture University, Japan.
• Agrobacterium & Biolistic- mediated transformation.
• Molecular characterization of transgenic plants using molecular biological techniques (southern, northern & western blotting, ELISA, ).
• Quantitative detection of proteins (post-translational modification), using radiolabeled amino acid incorporated into protein detection.
• Plasmid DNA isolation, and transformation of bacteria for preparation of transformation construct (vectors).
• Bacteria culturing, selection of transformed cells, maintenance of cultures.
• Genetically Modified Organisms (GMOs)- detection by nucleic acids & protein-based methods.
• Field evaluation of transgenic plants for agronomically important traits.

Faculty of Agriculture

Damascus University

MSc. Studies (2001/2003)
Project title: In Vitro micro-tuberization of "Draja" and some introduced varieties of potato using plant tissue culture techniques. Faculty of Agriculture, Damascus University, Syria.
• Tissue culture and micro-grafting techniques.
• Meristem isolation and culture.
• Regeneration survey (evaluation of plant genotypes for response to tissue culture).
• Standardization plant tissue culture protocol.
• Genomic DNA/RNA isolation, quantification, and qualification.
• DNA markers for crop improvement (RAPD, AFLP, RFLP, SSR, SRAP, SCAR marker).

Qualifications & Certifications

Engineering & Molecular Biology

Graduate School of Life & Environmental Sciences, Osaka Prefecture University

Agriculture

Damascus University

Field of Crops

Damascus University

Agricultural Engineering

Damascus University

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