David - Postdoctoral Researcher - Bowling Green, OH, USA

Justin David

Bowling Green, OH, USA


Postdoctoral Researcher


• Performed non invasive optical detection of arthritis progression in arthritic mouse models using fluorescent lipid-near infra-red optical probes studying its efficiency in identifying matrix-metallo proteinases involved in disease progression with whole animal optical imagers- IVIS series / LICOR Pearl (Time lapse Imaging)
• Performed mammalian cell culture, Transfection, designed Primers, PCR, cloning and DNA sequencing, technical expertise in mouse model, Tumor xenografts
• Lab instructor for Bioengineering Undergraduate courses on “Fundamental Techniques of Imaging” and “In vivo Fluorescence Imaging”

Work History

Postdoctoral researcher

University of Pennsylvania

April 2009 - April 2011

Devised, designed and executed research work on evaluating Novel optical imaging fluorescent probes in arthritic mouse model.

Designed and carried out non-invasive optical imaging methodologies for monitoring arthritic disease progression in mouse models

studied role of Phospholipase A2 in breast cancer cells in signalling and tested near infra red probes that could measure the overexpression of PLA2 in vitro

Xenografts implanted for studying NIR probes that could detect expression of MMPS in tumour and arthritic paws

Post Doctoral Researcher

University of Pennsylvania

April 2009 - April 2012

-Gained expertise in Optical imaging of MMPs using Xenogen's IVIS equipment

- Expertise in Non-invasive optical imaging of MMPs in arthritic mouse models employing novel synthesized fluorescent optical probes

- Raised and maintained cancer cell lines for studying the role of Phospholipases

-Expertise in xenografts and induction of arthritis protocols

-Served as Lab instructor for Bioengineering under graduate students in optical imaging methodologies

Postdoctoral researcher

University of Pennsylvania

May 2006 - April 2009

Designed and executed in vivo screening assays, primary and secondary to evaluate the effect of novel chemical compounds that could serve as lead compounds to drugs that inhibit lipid absorption/uptake

Studied effects of drugs like Ezetimibe and Atorvastatin in inhibiting lipid absorption in humans using zebrafish mouse model. Tested drugs that alter intestinal motility in humans such a L name, SNP etc.,

Carried out research to evaluate the effects of Schizophrenic drugs on impaired motility and lipid absorption.

Post Doc Researcher- Zebrafish High-throughput screening specialist

University of Pennsylvania

May 2006 - April 2009

- Pharmacological inhibition of dietary lipid absorption using zebrafish as model

- designed and executed Primary and Secondary in vivo screening assays for identifying novel compounds inhibiting lipid absorption

- tested and improvised methodologies for imaging a couple of engineered fluorescent optical reporters at sub cellular level

- worked with drugs like Atorvastatin, Ezetimibe and Orlistat

- gained expertise in handling fluorescent microscopy and confocal microscopy for imaging experiments involving fluorescent optical reporters




Doctor of Philosophy

Contributed to ploidy manipulation and by investigating possibilities of resurrecting a lost or endangered fish population and design protocols required to generate and test genomic contribution in androgenetic clones, triploids and tetraploids. Successfully generated androgenetic clones and introduced androgenesis (exclusively paternal inheritance) in a fish model suggesting the use of heterologous sperm for resurrecting an endangered species using genome-inactivated egg of another species. Designed and successfully executed cadaveric sperm cryopreservation by simple protocols to help save valuable brood stock and endangered fish population.
Successfully isolated and sequenced Sex-specific DMRT1 like sequences in fish model for earlier identification of sex in androgenetic clones and control progenies. Designed and executed RAPD analyses for identifying species-specific molecular markers in fish for early identification of species in androgenesis, triploidy and tetraploidy. Successfully screened for developmental synchronization in embryonic development among androgenotes, triploids and tetraploids. Tested the progenies until 3 generations to identify the role of maternal and paternal autosomes in sex determination.

Post Doctoral fellow - School of Medicine

Tested drugs used to treat obesity in humans such Orlistat, Atorvastatin and zetimibe in zebrafish model system and investigated occurrence of side effects upon long term exposure. Successfully established protocols and histological methodologies for understanding enterocyte lipid transport using AM 143 and fluorescent microscopic analysis of sub cellular localization of lipids using lipid biosensors. Designed and executed screening assays to detect modulators of intestinal motility in zebrafish employing fluorescently labeled probes. Designed and executed screening assays for identifying compounds similar to pentylene tetrazole in eliciting neuro-behavioural effects using zebrafish model system.

High-throughput Screening Specialist - Penn Center for Molecular Drug Discovery

Chemical genetics for identifying novel modulators of dietary lipid absorption: Designed and executed screening assays for identifying modulators of lipid absorption from a chemical library of compounds and validates their mechanism of action using subsequent secondary assays. Designed and executed in vivo high throughput screening methodologies and assays employing novel Engineered fluorescent lipid probes as biosensors for detecting dietary lipid absorption at sub cellular level in zebrafish model. Proficiency and expertise in designing primary and secondary assays for investigating lipid absorption.

Post Doctoral Researcher, Molecular Imaging, Radiology, Penn

Molecular imaging of Phospholipase activity in cancer cell lines

Designed and executed research in identifying role of phospholipases in tumor growth. Expertise in growing mammalian cell lines for testing NIRF probes that target Phospholipases in vitro and in vivo. Cloned and Sequenced Phospholipase A2 in cancer cell lines and studied their expression by confocal microscopy imaging. Related research investigated suitability of using engineered fluorescent molecular lipid probes in detecting expression of phospholipase in cancer cell lines

Post Doctoral Researcher

Molecular imaging of Matrix Metallo Proteinases (MMP) in Arthritis progression using Near Infra Red Fluorescent (NIRF) Probes
Improvised animal protocols for Induction of arthritis in KRN serum transfer mouse model. Improvised tail vein injection of probes and animal handling methodologies. Designed and executed whole animal In vivo imaging methodologies for detecting MMP's in arthritic mouse models using novel synthesized NIRF probes from Bracco Research, USA. Designed, and executed novel in vivo imaging assays using MMP probes and validated efficiency in detecting disease progression in mouse models. Designed and tested NIRF probes imaging and suitability for in vitro activation of synovial fluid using plate reader

Qualifications & Certifications

Doctorate in Biology - Molecular Genetics

Madurai Kamaraj University

Master of Philosophy

Madurai Kamaraj University

Master of Science

Madurai Kamaraj University

Seventh Day Adventist Higher Secondary School

Doctor of Philosophy (Ph.D.)

Madurai Kamaraj University

Master in Philosophy

Madurai Kamaraj University

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